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cell lines hek293t cells atcc crl3216 h vegf reporter 293 cell line genomeditech gm c09057 experimental models
Cell Lines Hek293t Cells Atcc Crl3216 H Vegf Reporter 293 Cell Line Genomeditech Gm C09057 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 37751 article reviews
Cell Lines Hek293t Cells Atcc Crl3216 H Vegf Reporter 293 Cell Line Genomeditech Gm C09057 Experimental Models, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 37751 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+lines+hek293t+atcc+crl/293T/pm42285094-518-7-11
Average 99 stars, based on 37751 article reviews
cell lines hek293t cells atcc crl3216 h vegf reporter 293 cell line genomeditech gm c09057 experimental models - by Bioz Stars,
2026-09
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Transfection:Article Title: ZAP inhibits double-stranded RNA virus infection by degrading negative-strand RNA and blocking the elongation phase of viral protein synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4% paraformaldehyde biosharp Cat#BL539A Cycloheximide MCE Cat#HY-12320 Harringtonine MCE Cat#HY-N0862 Lipofectamine 3000 Thermo Fisher Cat#L3000015 Lipofectamine TM RNAiMAX Transfection Reagent Thermo Fisher Cat#13778150 Triton X-100 Sigma-Aldrich Cat#9036-19-5 PMSF Solarbio Cat#P0100 Recombinant RNase Inhibitor Takara Cat#2313B DMSO Sigma-Aldrich Cat#D2650 Deposited data CLIP-seq This paper GSA: CRA031455 Original western blot images This paper https://doi.org/10.17632/x448sh5bh2.1 (Mendeley) Experimental models: Recombinant:Article Title: ZAP inhibits double-stranded RNA virus infection by degrading negative-strand RNA and blocking the elongation phase of viral protein synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4% paraformaldehyde biosharp Cat#BL539A Cycloheximide MCE Cat#HY-12320 Harringtonine MCE Cat#HY-N0862 Lipofectamine 3000 Thermo Fisher Cat#L3000015 Lipofectamine TM RNAiMAX Transfection Reagent Thermo Fisher Cat#13778150 Triton X-100 Sigma-Aldrich Cat#9036-19-5 PMSF Solarbio Cat#P0100 Recombinant RNase Inhibitor Takara Cat#2313B DMSO Sigma-Aldrich Cat#D2650 Deposited data CLIP-seq This paper GSA: CRA031455 Original western blot images This paper https://doi.org/10.17632/x448sh5bh2.1 (Mendeley) Experimental models: Cross-linking Immunoprecipitation:Article Title: ZAP inhibits double-stranded RNA virus infection by degrading negative-strand RNA and blocking the elongation phase of viral protein synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4% paraformaldehyde biosharp Cat#BL539A Cycloheximide MCE Cat#HY-12320 Harringtonine MCE Cat#HY-N0862 Lipofectamine 3000 Thermo Fisher Cat#L3000015 Lipofectamine TM RNAiMAX Transfection Reagent Thermo Fisher Cat#13778150 Triton X-100 Sigma-Aldrich Cat#9036-19-5 PMSF Solarbio Cat#P0100 Recombinant RNase Inhibitor Takara Cat#2313B DMSO Sigma-Aldrich Cat#D2650 Deposited data CLIP-seq This paper GSA: CRA031455 Original western blot images This paper https://doi.org/10.17632/x448sh5bh2.1 (Mendeley) Experimental models: Western Blot:Article Title: ZAP inhibits double-stranded RNA virus infection by degrading negative-strand RNA and blocking the elongation phase of viral protein synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4% paraformaldehyde biosharp Cat#BL539A Cycloheximide MCE Cat#HY-12320 Harringtonine MCE Cat#HY-N0862 Lipofectamine 3000 Thermo Fisher Cat#L3000015 Lipofectamine TM RNAiMAX Transfection Reagent Thermo Fisher Cat#13778150 Triton X-100 Sigma-Aldrich Cat#9036-19-5 PMSF Solarbio Cat#P0100 Recombinant RNase Inhibitor Takara Cat#2313B DMSO Sigma-Aldrich Cat#D2650 Deposited data CLIP-seq This paper GSA: CRA031455 Original western blot images This paper https://doi.org/10.17632/x448sh5bh2.1 (Mendeley) Experimental models: Real-time Polymerase Chain Reaction:Article Title: ZAP inhibits double-stranded RNA virus infection by degrading negative-strand RNA and blocking the elongation phase of viral protein synthesis. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 4% paraformaldehyde biosharp Cat#BL539A Cycloheximide MCE Cat#HY-12320 Harringtonine MCE Cat#HY-N0862 Lipofectamine 3000 Thermo Fisher Cat#L3000015 Lipofectamine TM RNAiMAX Transfection Reagent Thermo Fisher Cat#13778150 Triton X-100 Sigma-Aldrich Cat#9036-19-5 PMSF Solarbio Cat#P0100 Recombinant RNase Inhibitor Takara Cat#2313B DMSO Sigma-Aldrich Cat#D2650 Deposited data CLIP-seq This paper GSA: CRA031455 Original western blot images This paper https://doi.org/10.17632/x448sh5bh2.1 (Mendeley) Experimental models: Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: RNA Sequencing:Article Title: Out-of-frame CBX3::ALK fusion drives ALK activation and therapy response. Article Snippet: GRCh38.dna_sm.primary_assembly.fa.gz Ensembl Gene Transfer File release 110, GRCh38 Ensembl https://ftp.ensembl.org/pub/release-110/ gtf/homo_sapiens/Homo_sapiens. .. GRCh38.110.gtf.gz COSMIC (version 86) Forbes et al. https://cancer.sanger.ac.uk/cosmic/ download/cosmic Genome Aggregation Database (version v2.0.2) Karczewski et al. https://gnomad.broadinstitute.org/downloads TCGA RNA-seq bam files Wilks et al. https://gdc.cancer.gov/ RNA-seq bam files of the Australian melanoma cohort Newell et al. EGA: EGAD00001008837 Experimental models: Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: Electron Microscopy:Article Title: Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells. Article Snippet: Antibodies Mouse anti pH3-647 Abcam Cat# ab196698 Chemicals, peptides, and recombinant proteins Collagenase, from Clostridium histolyticum Sigma Cat# C2674 Dulbecco’s modified Eagle’s medium (DMEM) Gibco Cat# C11995500BT Fetal bovine serum (FBS) Gibco Cat# 10091-148; Lot# 2556131P Fetal bovine serum (heat inactivated) Sigma Cat# F4135 Glycine VWR Life Science Cat# 0167 Insulin solution from bovine pancreas Sigma Cat# I0516 L-glutamine solution Sigma Cat# G7513 L-glutathione reduced Sigma Cat# G6013 (Continued on next page) 2 STAR Protocols 6, 103858, June 20, 2025 .. MEM non-essential amino acids solution (1003) Gibco Cat# 11140-050 Papain, from papaya latex Sigma Cat# P4762 8% paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 157-8 Penicillin-streptomycin Gibco Cat# 15140-122 Poly-D-lysine Sigma Cat# P6282 Schneider Drosophila culture medium Gibco Cat# 21720024 Triton X-100 Sigma Cat# T8787 0.25% trypsin with EDTA Gibco Cat# 25200-072 VAHTS DNA clean beads Vazyme Cat# N411 Experimental models: Software:Article Title: Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells. Article Snippet: Antibodies Mouse anti pH3-647 Abcam Cat# ab196698 Chemicals, peptides, and recombinant proteins Collagenase, from Clostridium histolyticum Sigma Cat# C2674 Dulbecco’s modified Eagle’s medium (DMEM) Gibco Cat# C11995500BT Fetal bovine serum (FBS) Gibco Cat# 10091-148; Lot# 2556131P Fetal bovine serum (heat inactivated) Sigma Cat# F4135 Glycine VWR Life Science Cat# 0167 Insulin solution from bovine pancreas Sigma Cat# I0516 L-glutamine solution Sigma Cat# G7513 L-glutathione reduced Sigma Cat# G6013 (Continued on next page) 2 STAR Protocols 6, 103858, June 20, 2025 .. MEM non-essential amino acids solution (1003) Gibco Cat# 11140-050 Papain, from papaya latex Sigma Cat# P4762 8% paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 157-8 Penicillin-streptomycin Gibco Cat# 15140-122 Poly-D-lysine Sigma Cat# P6282 Schneider Drosophila culture medium Gibco Cat# 21720024 Triton X-100 Sigma Cat# T8787 0.25% trypsin with EDTA Gibco Cat# 25200-072 VAHTS DNA clean beads Vazyme Cat# N411 Experimental models: Cell Culture:Article Title: Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells. Article Snippet: Antibodies Mouse anti pH3-647 Abcam Cat# ab196698 Chemicals, peptides, and recombinant proteins Collagenase, from Clostridium histolyticum Sigma Cat# C2674 Dulbecco’s modified Eagle’s medium (DMEM) Gibco Cat# C11995500BT Fetal bovine serum (FBS) Gibco Cat# 10091-148; Lot# 2556131P Fetal bovine serum (heat inactivated) Sigma Cat# F4135 Glycine VWR Life Science Cat# 0167 Insulin solution from bovine pancreas Sigma Cat# I0516 L-glutamine solution Sigma Cat# G7513 L-glutathione reduced Sigma Cat# G6013 (Continued on next page) 2 STAR Protocols 6, 103858, June 20, 2025 .. MEM non-essential amino acids solution (1003) Gibco Cat# 11140-050 Papain, from papaya latex Sigma Cat# P4762 8% paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 157-8 Penicillin-streptomycin Gibco Cat# 15140-122 Poly-D-lysine Sigma Cat# P6282 Schneider Drosophila culture medium Gibco Cat# 21720024 Triton X-100 Sigma Cat# T8787 0.25% trypsin with EDTA Gibco Cat# 25200-072 VAHTS DNA clean beads Vazyme Cat# N411 Experimental models: Microscopy:Article Title: Protocol for identifying genomic binding sites of mitotic bookmarkers in Drosophila neural stem cells and cultured mammalian cells. Article Snippet: Antibodies Mouse anti pH3-647 Abcam Cat# ab196698 Chemicals, peptides, and recombinant proteins Collagenase, from Clostridium histolyticum Sigma Cat# C2674 Dulbecco’s modified Eagle’s medium (DMEM) Gibco Cat# C11995500BT Fetal bovine serum (FBS) Gibco Cat# 10091-148; Lot# 2556131P Fetal bovine serum (heat inactivated) Sigma Cat# F4135 Glycine VWR Life Science Cat# 0167 Insulin solution from bovine pancreas Sigma Cat# I0516 L-glutamine solution Sigma Cat# G7513 L-glutathione reduced Sigma Cat# G6013 (Continued on next page) 2 STAR Protocols 6, 103858, June 20, 2025 .. MEM non-essential amino acids solution (1003) Gibco Cat# 11140-050 Papain, from papaya latex Sigma Cat# P4762 8% paraformaldehyde (PFA) Electron Microscopy Sciences Cat# 157-8 Penicillin-streptomycin Gibco Cat# 15140-122 Poly-D-lysine Sigma Cat# P6282 Schneider Drosophila culture medium Gibco Cat# 21720024 Triton X-100 Sigma Cat# T8787 0.25% trypsin with EDTA Gibco Cat# 25200-072 VAHTS DNA clean beads Vazyme Cat# N411 Experimental models: other:Article Title: Library-based single-cell analysis of CAR signaling reveals drivers of in vivo persistence. Article Snippet: Article Article Title: Epigenetic control of microglial mitochondrial immunity by KAT7 drives Alzheimer's disease pathogenesis. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Matrigel Corning Cat #354230 Y-27632 StemCell Technologies Cat #72304 Doxycycline Sigma-Aldrich Cat #D9891 Critical commercial assays RNAscope Multiplex Fluorescent Reagent Kit v.2 Advanced Cell Diagnostics Cat #323100 TSA Plus Fluorescein Evaluation Kit PerkinElmer Cat #NEL741 Click-iT EdU Alexa Fluor 488 Imaging Kit Invitrogen Cat #C10337 HiScript III RT SuperMix for qPCR Kit Vazyme Cat #R323-01 Pierce BCA Protein Assay Kit Thermo Cat #23227 ZymoPURE II Plasmid Midiprep kit Zymo Research Cat #D4201 Mouse IL-6 ELISA Kits BioLegend Cat #431301 Mouse IL-1β ELISA Kits BioLegend Cat #432601 Human IL-6 ELISA Kits BioLegend Cat #430501 CUT&Tag Assay Kit Vazyme Cat #TD904 TruePrep Index Kit V2 for Illumina Vazyme Cat #TD202 CD11b MicroBeads Miltenyi Biotec Cat #130-093-634 Osmotic pumps RWD Cat #1004W Brain infusion kit RWD Cat #Bic-3 Deposited Article Title: Pharmacologic interrogation of USP28 cellular function in p53 signaling. Article Snippet: Article Pharmacologic interrogation of USP28 cellular function in p53 signaling Protease Inhibitor:Article Title: MAP2K6 directly phosphorylates BCL2L13 to mediate mitophagy for suppressing tumorigenicity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ACTIN antibody Sigma-Aldrich Cat#A2228; RRID:AB_476697 Anti-MKK3 antibody Abcam Cat#ab195037; RRID:AB_3675522 Anti-MKK6 antibody Abcam Cat#ab154901 An-MKK6 antibody Cell Signaling Technology Cat#8550; RRID:AB_11220227 Anti-LC3B Cell Signaling Technology Cat#83506; RRID:AB_2800018 Anti-TIMM23 Abcam Cat#ab230253; RRID:AB_3697281 Anti-HSP60 Abcam Cat#ab46798; RRID:AB_881444 Anti-TOMM20 Abcam Cat#ab56783; RRID:AB_945896 Anti-BCL2L13 Proteintech Cat#16612-1-AP; RRID:AB_1850928 Anti-p38 Cell Signaling Technology Cat#8690; RRID:AB_10999090 Anti-p-p38 Cell Signaling Technology Cat#9211; RRID:AB_331641 Anti-ATG5 Cell Signaling Technology Cat#12994; RRID:AB_2630393 Anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Anti-His Cell Signaling Technology Cat#12698; RRID:AB_2744546 Anti-Flag Sigma-Aldrich Cat#F1804; RRID:AB_262044 Anti-p-Ser Santa Cruz Cat#sc-81514; RRID:AB_1128624 Anti-GST ABclonal Cat#AE001; RRID:AB_2770403 Anti-Ki67 Cell Signaling Technology Cat#34330; RRID:AB_2942026 HRP-conjugated goat anti-mouse antibody Kangchen Cat#KC-MM-035; RRID:AB_2665472 HRP-conjugated goat anti-rabbit antibody Kangchen Cat#KC-RB-035 Goat anti-Mouse IgG (H + L), Alexa Fluor 488 Invitrogen Cat#A11001; RRID:AB_2534069 Goat anti-Rabbit IgG (H + L), Alexa Fluor 568 Invitrogen Cat#A11011; RRID:AB_143157 Bacterial and virus strains E. coli BL21 Lab stored N/A E. coli Stabl3 Lab stored N/A E. coli DH5a Lab stored N/A Adenovirus (Ad-Cre) Hanheng Biotechnology (Shanghai, China) N/A Adeno-associated virus (AAV-3Flag/MKK33Flag/MKK6-3Flag) Hanheng Biotechnology (Shanghai, China) N/A Biological samples Human LUAD tissues and adjacent normal lung Prof. Wenhua Liang (First Affiliated Hospital of Guangzhou Medical University) N/A Chemicals, peptides, and recombinant proteins GlutaMAX GIBCO Cat#35050079 Non-Essential Amino Acids Solution (NEAA) GIBCO Cat#11140076 DMEM GIBCO Cat#11995500BT Fetal Bovine Serum GIBCO Cat#10099141 EBSS medium GIBCO Cat#2401004 3-Methyladenine (3-MA) Selleck Cat#S2767 Bafilomycin A1 (BAF) Selleck Cat# S1413 PMSF Beyotime Cat#ST506 Triton X-100 Sigma Cat#T8787 (Continued on next page) 18 Cell Reports 45, 117177, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER protease inhibitor cocktail Selleck Cat#B14001 RIPA Beyotime Cat#P0013B phosphatase inhibitor cocktail Beyotime Cat#B15001 NP-40 buffer Beyotime Cat#P0013F CK1 buffer NEB Cat#B6030S Flag peptide Beyotime Cat#P9801 HA peptide ThermoFisher Cat#88836 Critical commercial assays Genomic DNA Mini Preparation Kit Beyotime Cat#D0063 Mini Plasmid Kit TIANGEN Cat#W9524 Anti-HA Magnetic Beads ThermoFisher Cat#88836 PierceTM Anti-DYKDDDDK Magnetic Agarose ThermoFisher Cat#A36797 Dynabeads with protein A Invitrogen Cat#10002D Dynabeads with protein G Invitrogen Cat#10004D The Immunohistochemical secondary antibody kit instructions Absin Bioscience Inc Cat#abs996 Duolink®In Situ Red Starter Kit Mouse/ Rabbit Sigma-Aldrich Cat#DUO92101-1KT Deposited data Raw MS data of proteomic or phosphoproteomic This manuscript ProteomeXchange: https:// proteomecentral.proteomexchange.org/ ?pxid=PXD074976 Experimental models: Plasmid Preparation:Article Title: MAP2K6 directly phosphorylates BCL2L13 to mediate mitophagy for suppressing tumorigenicity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ACTIN antibody Sigma-Aldrich Cat#A2228; RRID:AB_476697 Anti-MKK3 antibody Abcam Cat#ab195037; RRID:AB_3675522 Anti-MKK6 antibody Abcam Cat#ab154901 An-MKK6 antibody Cell Signaling Technology Cat#8550; RRID:AB_11220227 Anti-LC3B Cell Signaling Technology Cat#83506; RRID:AB_2800018 Anti-TIMM23 Abcam Cat#ab230253; RRID:AB_3697281 Anti-HSP60 Abcam Cat#ab46798; RRID:AB_881444 Anti-TOMM20 Abcam Cat#ab56783; RRID:AB_945896 Anti-BCL2L13 Proteintech Cat#16612-1-AP; RRID:AB_1850928 Anti-p38 Cell Signaling Technology Cat#8690; RRID:AB_10999090 Anti-p-p38 Cell Signaling Technology Cat#9211; RRID:AB_331641 Anti-ATG5 Cell Signaling Technology Cat#12994; RRID:AB_2630393 Anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Anti-His Cell Signaling Technology Cat#12698; RRID:AB_2744546 Anti-Flag Sigma-Aldrich Cat#F1804; RRID:AB_262044 Anti-p-Ser Santa Cruz Cat#sc-81514; RRID:AB_1128624 Anti-GST ABclonal Cat#AE001; RRID:AB_2770403 Anti-Ki67 Cell Signaling Technology Cat#34330; RRID:AB_2942026 HRP-conjugated goat anti-mouse antibody Kangchen Cat#KC-MM-035; RRID:AB_2665472 HRP-conjugated goat anti-rabbit antibody Kangchen Cat#KC-RB-035 Goat anti-Mouse IgG (H + L), Alexa Fluor 488 Invitrogen Cat#A11001; RRID:AB_2534069 Goat anti-Rabbit IgG (H + L), Alexa Fluor 568 Invitrogen Cat#A11011; RRID:AB_143157 Bacterial and virus strains E. coli BL21 Lab stored N/A E. coli Stabl3 Lab stored N/A E. coli DH5a Lab stored N/A Adenovirus (Ad-Cre) Hanheng Biotechnology (Shanghai, China) N/A Adeno-associated virus (AAV-3Flag/MKK33Flag/MKK6-3Flag) Hanheng Biotechnology (Shanghai, China) N/A Biological samples Human LUAD tissues and adjacent normal lung Prof. Wenhua Liang (First Affiliated Hospital of Guangzhou Medical University) N/A Chemicals, peptides, and recombinant proteins GlutaMAX GIBCO Cat#35050079 Non-Essential Amino Acids Solution (NEAA) GIBCO Cat#11140076 DMEM GIBCO Cat#11995500BT Fetal Bovine Serum GIBCO Cat#10099141 EBSS medium GIBCO Cat#2401004 3-Methyladenine (3-MA) Selleck Cat#S2767 Bafilomycin A1 (BAF) Selleck Cat# S1413 PMSF Beyotime Cat#ST506 Triton X-100 Sigma Cat#T8787 (Continued on next page) 18 Cell Reports 45, 117177, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER protease inhibitor cocktail Selleck Cat#B14001 RIPA Beyotime Cat#P0013B phosphatase inhibitor cocktail Beyotime Cat#B15001 NP-40 buffer Beyotime Cat#P0013F CK1 buffer NEB Cat#B6030S Flag peptide Beyotime Cat#P9801 HA peptide ThermoFisher Cat#88836 Critical commercial assays Genomic DNA Mini Preparation Kit Beyotime Cat#D0063 Mini Plasmid Kit TIANGEN Cat#W9524 Anti-HA Magnetic Beads ThermoFisher Cat#88836 PierceTM Anti-DYKDDDDK Magnetic Agarose ThermoFisher Cat#A36797 Dynabeads with protein A Invitrogen Cat#10002D Dynabeads with protein G Invitrogen Cat#10004D The Immunohistochemical secondary antibody kit instructions Absin Bioscience Inc Cat#abs996 Duolink®In Situ Red Starter Kit Mouse/ Rabbit Sigma-Aldrich Cat#DUO92101-1KT Deposited data Raw MS data of proteomic or phosphoproteomic This manuscript ProteomeXchange: https:// proteomecentral.proteomexchange.org/ ?pxid=PXD074976 Experimental models: Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: Magnetic Beads:Article Title: MAP2K6 directly phosphorylates BCL2L13 to mediate mitophagy for suppressing tumorigenicity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ACTIN antibody Sigma-Aldrich Cat#A2228; RRID:AB_476697 Anti-MKK3 antibody Abcam Cat#ab195037; RRID:AB_3675522 Anti-MKK6 antibody Abcam Cat#ab154901 An-MKK6 antibody Cell Signaling Technology Cat#8550; RRID:AB_11220227 Anti-LC3B Cell Signaling Technology Cat#83506; RRID:AB_2800018 Anti-TIMM23 Abcam Cat#ab230253; RRID:AB_3697281 Anti-HSP60 Abcam Cat#ab46798; RRID:AB_881444 Anti-TOMM20 Abcam Cat#ab56783; RRID:AB_945896 Anti-BCL2L13 Proteintech Cat#16612-1-AP; RRID:AB_1850928 Anti-p38 Cell Signaling Technology Cat#8690; RRID:AB_10999090 Anti-p-p38 Cell Signaling Technology Cat#9211; RRID:AB_331641 Anti-ATG5 Cell Signaling Technology Cat#12994; RRID:AB_2630393 Anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Anti-His Cell Signaling Technology Cat#12698; RRID:AB_2744546 Anti-Flag Sigma-Aldrich Cat#F1804; RRID:AB_262044 Anti-p-Ser Santa Cruz Cat#sc-81514; RRID:AB_1128624 Anti-GST ABclonal Cat#AE001; RRID:AB_2770403 Anti-Ki67 Cell Signaling Technology Cat#34330; RRID:AB_2942026 HRP-conjugated goat anti-mouse antibody Kangchen Cat#KC-MM-035; RRID:AB_2665472 HRP-conjugated goat anti-rabbit antibody Kangchen Cat#KC-RB-035 Goat anti-Mouse IgG (H + L), Alexa Fluor 488 Invitrogen Cat#A11001; RRID:AB_2534069 Goat anti-Rabbit IgG (H + L), Alexa Fluor 568 Invitrogen Cat#A11011; RRID:AB_143157 Bacterial and virus strains E. coli BL21 Lab stored N/A E. coli Stabl3 Lab stored N/A E. coli DH5a Lab stored N/A Adenovirus (Ad-Cre) Hanheng Biotechnology (Shanghai, China) N/A Adeno-associated virus (AAV-3Flag/MKK33Flag/MKK6-3Flag) Hanheng Biotechnology (Shanghai, China) N/A Biological samples Human LUAD tissues and adjacent normal lung Prof. Wenhua Liang (First Affiliated Hospital of Guangzhou Medical University) N/A Chemicals, peptides, and recombinant proteins GlutaMAX GIBCO Cat#35050079 Non-Essential Amino Acids Solution (NEAA) GIBCO Cat#11140076 DMEM GIBCO Cat#11995500BT Fetal Bovine Serum GIBCO Cat#10099141 EBSS medium GIBCO Cat#2401004 3-Methyladenine (3-MA) Selleck Cat#S2767 Bafilomycin A1 (BAF) Selleck Cat# S1413 PMSF Beyotime Cat#ST506 Triton X-100 Sigma Cat#T8787 (Continued on next page) 18 Cell Reports 45, 117177, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER protease inhibitor cocktail Selleck Cat#B14001 RIPA Beyotime Cat#P0013B phosphatase inhibitor cocktail Beyotime Cat#B15001 NP-40 buffer Beyotime Cat#P0013F CK1 buffer NEB Cat#B6030S Flag peptide Beyotime Cat#P9801 HA peptide ThermoFisher Cat#88836 Critical commercial assays Genomic DNA Mini Preparation Kit Beyotime Cat#D0063 Mini Plasmid Kit TIANGEN Cat#W9524 Anti-HA Magnetic Beads ThermoFisher Cat#88836 PierceTM Anti-DYKDDDDK Magnetic Agarose ThermoFisher Cat#A36797 Dynabeads with protein A Invitrogen Cat#10002D Dynabeads with protein G Invitrogen Cat#10004D The Immunohistochemical secondary antibody kit instructions Absin Bioscience Inc Cat#abs996 Duolink®In Situ Red Starter Kit Mouse/ Rabbit Sigma-Aldrich Cat#DUO92101-1KT Deposited data Raw MS data of proteomic or phosphoproteomic This manuscript ProteomeXchange: https:// proteomecentral.proteomexchange.org/ ?pxid=PXD074976 Experimental models: Immunohistochemical staining:Article Title: MAP2K6 directly phosphorylates BCL2L13 to mediate mitophagy for suppressing tumorigenicity. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-ACTIN antibody Sigma-Aldrich Cat#A2228; RRID:AB_476697 Anti-MKK3 antibody Abcam Cat#ab195037; RRID:AB_3675522 Anti-MKK6 antibody Abcam Cat#ab154901 An-MKK6 antibody Cell Signaling Technology Cat#8550; RRID:AB_11220227 Anti-LC3B Cell Signaling Technology Cat#83506; RRID:AB_2800018 Anti-TIMM23 Abcam Cat#ab230253; RRID:AB_3697281 Anti-HSP60 Abcam Cat#ab46798; RRID:AB_881444 Anti-TOMM20 Abcam Cat#ab56783; RRID:AB_945896 Anti-BCL2L13 Proteintech Cat#16612-1-AP; RRID:AB_1850928 Anti-p38 Cell Signaling Technology Cat#8690; RRID:AB_10999090 Anti-p-p38 Cell Signaling Technology Cat#9211; RRID:AB_331641 Anti-ATG5 Cell Signaling Technology Cat#12994; RRID:AB_2630393 Anti-HA Cell Signaling Technology Cat#3724; RRID:AB_1549585 Anti-His Cell Signaling Technology Cat#12698; RRID:AB_2744546 Anti-Flag Sigma-Aldrich Cat#F1804; RRID:AB_262044 Anti-p-Ser Santa Cruz Cat#sc-81514; RRID:AB_1128624 Anti-GST ABclonal Cat#AE001; RRID:AB_2770403 Anti-Ki67 Cell Signaling Technology Cat#34330; RRID:AB_2942026 HRP-conjugated goat anti-mouse antibody Kangchen Cat#KC-MM-035; RRID:AB_2665472 HRP-conjugated goat anti-rabbit antibody Kangchen Cat#KC-RB-035 Goat anti-Mouse IgG (H + L), Alexa Fluor 488 Invitrogen Cat#A11001; RRID:AB_2534069 Goat anti-Rabbit IgG (H + L), Alexa Fluor 568 Invitrogen Cat#A11011; RRID:AB_143157 Bacterial and virus strains E. coli BL21 Lab stored N/A E. coli Stabl3 Lab stored N/A E. coli DH5a Lab stored N/A Adenovirus (Ad-Cre) Hanheng Biotechnology (Shanghai, China) N/A Adeno-associated virus (AAV-3Flag/MKK33Flag/MKK6-3Flag) Hanheng Biotechnology (Shanghai, China) N/A Biological samples Human LUAD tissues and adjacent normal lung Prof. Wenhua Liang (First Affiliated Hospital of Guangzhou Medical University) N/A Chemicals, peptides, and recombinant proteins GlutaMAX GIBCO Cat#35050079 Non-Essential Amino Acids Solution (NEAA) GIBCO Cat#11140076 DMEM GIBCO Cat#11995500BT Fetal Bovine Serum GIBCO Cat#10099141 EBSS medium GIBCO Cat#2401004 3-Methyladenine (3-MA) Selleck Cat#S2767 Bafilomycin A1 (BAF) Selleck Cat# S1413 PMSF Beyotime Cat#ST506 Triton X-100 Sigma Cat#T8787 (Continued on next page) 18 Cell Reports 45, 117177, April 28, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER protease inhibitor cocktail Selleck Cat#B14001 RIPA Beyotime Cat#P0013B phosphatase inhibitor cocktail Beyotime Cat#B15001 NP-40 buffer Beyotime Cat#P0013F CK1 buffer NEB Cat#B6030S Flag peptide Beyotime Cat#P9801 HA peptide ThermoFisher Cat#88836 Critical commercial assays Genomic DNA Mini Preparation Kit Beyotime Cat#D0063 Mini Plasmid Kit TIANGEN Cat#W9524 Anti-HA Magnetic Beads ThermoFisher Cat#88836 PierceTM Anti-DYKDDDDK Magnetic Agarose ThermoFisher Cat#A36797 Dynabeads with protein A Invitrogen Cat#10002D Dynabeads with protein G Invitrogen Cat#10004D The Immunohistochemical secondary antibody kit instructions Absin Bioscience Inc Cat#abs996 Duolink®In Situ Red Starter Kit Mouse/ Rabbit Sigma-Aldrich Cat#DUO92101-1KT Deposited data Raw MS data of proteomic or phosphoproteomic This manuscript ProteomeXchange: https:// proteomecentral.proteomexchange.org/ ?pxid=PXD074976 Experimental models: Modification:Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: Methylation:Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: Activity Assay:Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: RNA sequencing:Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: Knock-Out:Article Title: IGF2BP3 redirects glycolytic flux to promote one-carbon metabolism and RNA methylation. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Biotin anti-mouse/human CD45R/B220 Antibody, 500 ug Biolegend 103204; RRID: AB_312989 Biotin anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody, 500ug Biolegend 108404; RRID: AB_313369 Biotin anti-mouse NK-1.1 Antibody, 500 ug Biolegend 108704; RRID: AB_313391 Biotin anti-mouse TER-119/Erythroid Cells Antibody, 500ug Biolegend 116204; RRID: AB_313705 Biotin anti-mouse TCR β chain Antibody, 500ug Biolegend 109204; RRID: AB_313427 Biotin anti-mouse CD8a Antibody, 500ug Biolegend 100704; RRID: AB_312743 PE/Cyanine7 anti-mouse CD16/32 Antibody, 100ug Biolegend 101318; RRID: AB_2104156 eBioscienceTM Streptavidin eFluorTM 450 Conjugate LifeTech 48-4317-82; RRID: AB_10359737 CD34 Monoclonal Antibody (RAM34), Alexa Fluor 700, eBioscienceTM, 100ug LifeTech 56-0341-82; RRID: AB_493998 BD HorizonTM BV605 Mouse Anti-Mouse CD45.2 BD Biosciences 563051; RRID: AB_2737974 Bacterial strains 5-alpha Competent E. coli Thermo Fisher Scientific Cat# EC0112 Chemicals, peptides & reagents L-Glutamine (200mM) Thermo Fisher Scientific Cat# 25030-081 13C6-Glucose Cambridge Isotope Laboratories, Inc. Cat# CLM-1396 Halt protease inhibitor cocktail Thermo Fisher Scientific Cat# 78442 DPBS Thermo Fisher Scientific Cat# 14040117 DL-Norvaline Sigma-Aldrich N7502 G418 Thermo Fisher Scientific Cat# 11811-031 Cycloheximide Sigma-Aldrich Cat# 01810 Recombinant Human Flt3-Ligand Gibco Cat# PHC9415 Recombinant mouse SCF Gibco Cat# 25003100UG Recombinant mouse TPO Gibco Cat# 31514100UG Recombinant mouse IL-6 Gibco Cat# PHC0065 Puromycin dihydrochloride Sigma-Aldrich Cat# P4512 Penicillin Streptomycin Thermo Fisher Scientific Cat# 15-140-122 Halt Protease and Phosphotase inhibitors cocktail 100x Thermo Fisher Scientific Cat# PI-78440 SuperSignal West Pico PLUS substrate Thermo Fisher Scientific Cat# 34580 Streptavidin MicroBeads Miltenyi Biotec Cat# 130-048-101 Busulfan Sigma-Aldrich Cat# B2635 I3IN-002 Lab stock Recipe available upon request STM2457 Selleckchem Cat# S7890 DMSO Sigma-Aldrich Cat# D2650 RIPA Buffer Thermo Fisher Scientific Cat# NC9484499 TRI Reagent Zymo Research Cat# R2050 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 BioT DNA Transfection Reagent Bioland Cat# B01-01 PerfeCTa® SYBR® Green FastMix®, ROXTM VWR Cat# 101414-278 qScriptTM cDNA SuperMix VWR Cat# 101414-106 Fetal Bovine Serum, dialyzed, US origin, One ShotTM format Thermo Fisher Scientific Cat# A3382001 (Continued on next page) Cell Reports ▪▪, 116330, ▪▪, 2025 17 Article ll OPEN ACCESS .. REAGENT or RESOURCE SOURCE IDENTIFIER IMDM (Iscove’s Modified Dulbecco’s Medium) Thermo Fisher Scientific Cat# 12440053 DMEM, high glucose Thermo Fisher Scientific Cat# 11-995-073 RPMI 1640 Medium Thermo Fisher Scientific Cat# 11-875-093 DMEM, no glucose, no glutamine, no phenol red Thermo Fisher Scientific Cat# A1443001 Commercial assay kits Thermo ScientificTM PierceTM BCATM Protein Assay Thermo Fisher Scientific Cat# PI23225 MethoCult colony-forming media STEMCELL Technologies Cat# M3434 Quick-DNA/RNA Viral Magbead kit Zymo Research Cat# R2140 Luna Universal qPCR Master Mix NEB Cat# M3003 CellTiter-Glo(R) Luminescent Cell Viability Promega Cat# G7572 EpiQuick m6A RNA Methylation Quantification Kit Epigentek Cat# P-9005 Epigenase m6A Demethylase Activity/Inhibition Assay Kit Epigentek Cat# P-9013 Epigenase m6A Methylase Activity/Inhibition Assay Kit Epigentek Cat# P-9019 QIAGEN Plasmid Maxi Kit QIAGEN Cat# 12362 NEXT- FLEX Rapid Directional RNA-Seq Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-5198-01 NEXTFLEX Poly(A) Beads Kit 2.0 Perkin Elmer Applied Genomics Cat# NOVA-512991 Deposit data RNA-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 m6A IGF2BP3 KO (sg2) eCLIP-seq (Raw data) This study BioProject: PRJNA1191523 IGF2BP3 knockout (Metabolomics (LC/MS) Raw data) This study NMDR: PR002225 I3IN-002 treatment (Metabolomics (LC/MS) Raw data) This study NMDR: PR002475 Metabolomics (LC/MS) Analyzed data This study Table S1 Experimental models: |